Evaluation of whole blood profile as a tool in COVID-19 diagnosis and screening. A cross-sectional study

Autores/as

  • Nicolle Godoy Moreira aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Thaciane Alkmim Bibo aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Ana Carolina Macedo Gaiatto aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Joyce Regina Santos Raimundo aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Jéssica Freitas Araújo Encinas aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Beatriz da Costa Aguiar Alves aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Thaís Moura Gascón aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Fernando Luiz Affonso Fonseca aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil
  • Glaucia Luciano da Veiga aLaboratório de Análises Clínicas - Centro Universitário FMABC – Santo André, Brazil

DOI:

https://doi.org/10.36311/jhgd.v34.15750

Palabras clave:

Hematological profile, Hematimetric index, COVID-19, pandemic, screening

Resumen

Backgroung: the COVID-19 epidemic began in December 2019, and the shortage of diagnostic resources has affected the reported data on the number of cases, resulting in variations in reported cases between countries. This situation underscores the necessity for a deeper understanding of SARS-CoV-2 pathophysiology, including blood profiles and potential predictors.

Methods: hematological variables were studied in 200 patients diagnosed with COVID-19, before the vaccination period started. We analyzed hemogram parameters: erythrocytes, hemoglobin, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV) mean corpuscular hemoglobin concentration (MCHC), hematocrit, red cell distribution width (RDW), platelets, mean platelet volume (MPV), leukocytes, neutrophils, lymphocytes, neutrophils/lymphocytes ratio and Platelet-to-Lymphocyte Ratio (PLR), up to 9 days after positive result for COVID-19.

Results: the positive COVID-19 group presented a higher mean age, as well as a higher frequency of male individuals. Erythrocyte, hemoglobin, hematocrit and MCH values were significantly lower, while RDW and PLR showed higher values in the positive group. Leukocytes, neutrophil and the neutrophil/lymphocyte ratio presented higher values in the positive COVID-19 group.

Conclusion: data showed that the hemogram, a low-cost, minimally invasive exam, supports diagnosis and screening of COVID-19, allowing better evaluation of the disease course and assisting medical decisions facing lack of resources in a pandemic situation.

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Referencias

Ge H, Wang X, Yuan X, Xiao G, Wang C, Deng T, et al. The epidemiology and clinical information about COVID-19. Eur J Clin Microbiol Infect Dis 2020;39:1011–9. https://doi.org/10.1007/s10096-020-03874-z.

Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet 2020;395:497–506. https://doi.org/10.1016/S0140-6736(20)30183-5.

Dechamps M, De Poortere J, Martin M, Gatto L, Daumerie A, Bouzin C, et al. Inflammation-Induced Coagulopathy Substantially Differs Between COVID-19 and Septic Shock: A Prospective Observational Study. Front Med 2021;8:780750. https://doi.org/10.3389/fmed.2021.780750.

Organização Mundial de Saúde declara pandemia do novo Coronavírus n.d. https://www.unasus.gov.br/noticia/organizacao-mundial-de-saude-declara-pandemia-de-coronavirus

COVID-19 Map. Johns Hopkins Coronavirus Resource Center n.d. https://coronavirus.jhu.edu/map.html

Baud D, Qi X, Nielsen-Saines K, Musso D, Pomar L, Favre G. Real estimates of mortality following COVID-19 infection. Lancet Infect Dis 2020;20:773. https://doi.org/10.1016/S1473-3099(20)30195-X.

Morimont L, Dechamps M, David C, Bouvy C, Gillot C, Haguet H, et al. NETosis and Nucleosome Biomarkers in Septic Shock and Critical COVID-19 Patients: An Observational Study. Biomolecules 2022;12. https://doi.org/10.3390/biom12081038.

Ding X, Yu Y, Lu B, Huo J, Chen M, Kang Y, et al. Dynamic profile and clinical implications of hematological parameters in hospitalized patients with coronavirus disease 2019. Clin Chem Lab Med 2020;58:1365–71. https://doi.org/10.1515/cclm-2020-0411.

Yahav D. Hematological Aspects of COVID-19. Acta Haematol 2022;145:233–4. https://doi.org/10.1159/000524047.

Guan W-J, Ni Z-Y, Hu Y, Liang W-H, Ou C-Q, He J-X, et al. Clinical Characteristics of Coronavirus Disease 2019 in China. N Engl J Med 2020;382:1708–20. https://doi.org/10.1056/NEJMoa2002032.

Frater JL, Zini G, d’Onofrio G, Rogers HJ. COVID-19 and the clinical hematology laboratory. Int J Lab Hematol 2020;42 Suppl 1:11–8. https://doi.org/10.1111/ijlh.13229.

Yin Y, Wunderink RG. MERS, SARS and other coronaviruses as causes of pneumonia. Respirology 2018;23:130–7. https://doi.org/10.1111/resp.13196.

Zhang B, Zhou X, Zhu C, Song Y, Feng F, Qiu Y, et al. Immune Phenotyping Based on the Neutrophil-to-Lymphocyte Ratio and IgG Level Predicts Disease Severity and Outcome for Patients With COVID-19. Front Mol Biosci 2020;7:157. https://doi.org/10.3389/fmolb.2020.00157.

Chen R, Sang L, Jiang M, Yang Z, Jia N, Fu W, et al. Longitudinal hematologic and immunologic variations associated with the progression of COVID-19 patients in China. J Allergy Clin Immunol 2020;146:89–100. https://doi.org/10.1016/j.jaci.2020.05.003.

Henry BM, de Oliveira MHS, Benoit S, Plebani M, Lippi G. Hematologic, biochemical and immune biomarker abnormalities associated with severe illness and mortality in coronavirus disease 2019 (COVID-19): a meta-analysis. Clin Chem Lab Med 2020;58:1021–8. https://doi.org/10.1515/cclm-2020-0369.

Foy BH, Carlson JCT, Reinertsen E, Padros I Valls R, Pallares Lopez R, Palanques-Tost E, et al. Association of Red Blood Cell Distribution Width With Mortality Risk in Hospitalized Adults With SARS-CoV-2 Infection. JAMA Netw Open 2020;3:e2022058. https://doi.org/10.1001/jamanetworkopen.2020.22058.

de Oliveira Muniz Junior R, Lourenço P. Alterações laboratoriais e a COVID-19. Rev Bras Anal Clin 2020;52. https://doi.org/10.21877/2448-3877.20200013.

Lee JJ, Montazerin SM, Jamil A, Jamil U, Marszalek J, Chuang ML, et al. Association between red blood cell distribution width and mortality and severity among patients with COVID-19: A systematic review and meta-analysis. J Med Virol 2021;93:2513–22. https://doi.org/10.1002/jmv.26797.

Ramachandran P, Gajendran M, Perisetti A, Elkholy KO, Chakraborti A, Lippi G, et al. Red Blood Cell Distribution Width in Hospitalized COVID-19 Patients. Front Med 2021;8:582403. https://doi.org/10.3389/fmed.2021.582403.

Bergamaschi G, Borrelli de Andreis F, Aronico N, Lenti MV, Barteselli C, Merli S, et al. Anemia in patients with Covid-19: pathogenesis and clinical significance. Clin Exp Med 2021;21:239–46. https://doi.org/10.1007/s10238-020-00679-4.

Takeuchi H, Abe M, Takumi Y, Hashimoto T, Miyawaki M, Okamoto T, et al. Elevated red cell distribution width to platelet count ratio predicts poor prognosis in patients with breast cancer. Sci Rep 2019;9:3033. https://doi.org/10.1038/s41598-019-40024-8.

Voogt C, Smit K, Kleinjan M, Otten R, Scheffers T, Kuntsche E. From Age 4 to 8, Children Become Increasingly Aware About Normative Situations for Adults to Consume Alcohol. Alcohol Alcohol 2020;55:104–11. https://doi.org/10.1093/alcalc/agz093.

Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, et al. Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus-Infected Pneumonia. N Engl J Med 2020;382:1199–207. https://doi.org/10.1056/NEJMoa2001316.

Nordin G, Mårtensson A, Swolin B, Sandberg S, Christensen NJ, Thorsteinsson V, et al. A multicentre study of reference intervals for haemoglobin, basic blood cell counts and erythrocyte indices in the adult population of the Nordic countries. Scand J Clin Lab Invest 2004;64:385–98. https://doi.org/10.1080/00365510410002797.

Malka R, Brugnara C, Cialic R, Higgins JM. Non-Parametric Combined Reference Regions and Prediction of Clinical Risk. Clin Chem 2020;66:363–72. https://doi.org/10.1093/clinchem/hvz020.

Mishra P, Pandey CM, Singh U, Keshri A, Sabaretnam M. Selection of appropriate statistical methods for data analysis. Ann Card Anaesth 2019;22:297–301. https://doi.org/10.4103/aca.ACA_248_18.

Karampitsakos T, Akinosoglou K, Papaioannou O, Panou V, Koromilias A, Bakakos P, et al. Increased Red Cell Distribution Width Is Associated With Disease Severity in Hospitalized Adults With SARS-CoV-2 Infection: An Observational Multicentric Study. Front Med 2020;7:616292. https://doi.org/10.3389/fmed.2020.616292.

Lippi G, Plebani M. The critical role of laboratory medicine during coronavirus disease 2019 (COVID-19) and other viral outbreaks. Clin Chem Lab Med 2020;58:1063–9. https://doi.org/10.1515/cclm-2020-0240.

Brandão SCS, Godoi ETAM, Ramos J de OX, de Melo LMMP, Sarinho ESC. Severe COVID-19: understanding the role of immunity, endothelium, and coagulation in clinical practice. J Vasc Bras 2020;19:e20200131. https://doi.org/10.1590/1677-5449.200131.

Korniluk A, Koper-Lenkiewicz OM, Kamińska J, Kemona H, Dymicka-Piekarska V. Mean Platelet Volume (MPV): New Perspectives for an Old Marker in the Course and Prognosis of Inflammatory Conditions. Mediators Inflamm 2019;2019:9213074. https://doi.org/10.1155/2019/9213074.

Kim DS, Shin D, Lee MS, Kim HJ, Kim DY, Kim SM, et al. Assessments of neutrophil to lymphocyte ratio and platelet to lymphocyte ratio in Korean patients with psoriasis vulgaris and psoriatic arthritis. J Dermatol 2016;43:305–10. https://doi.org/10.1111/1346-8138.13061.

Qin B, Ma N, Tang Q, Wei T, Yang M, Fu H, et al. Neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) were useful markers in assessment of inflammatory response and disease activity in SLE patients. Mod Rheumatol 2016;26:372–6. https://doi.org/10.3109/14397595.2015.1091136.

Sarkar S, Kannan S, Khanna P, Singh AK. Role of platelet-to-lymphocyte count ratio (PLR), as a prognostic indicator in COVID-19: A systematic review and meta-analysis. J Med Virol 2022;94:211–21. https://doi.org/10.1002/jmv.27297.

Erdogan A, Can FE, Gönüllü H. Evaluation of the prognostic role of NLR, LMR, PLR, and LCR ratio in COVID-19 patients. J Med Virol 2021;93:5555–9. https://doi.org/10.1002/jmv.27097.

Aksu Y, Uslu AU, Tarhan G, Karagülle M. Predictive value of platelet to lymphocyte ratio and neutrophil to lymphocyte ratio in evaluating both lung involvement and severity of patients with coronavirus disease 2019. Saudi Med J 2021;42:1223–8. https://doi.org/10.15537/smj.2021.42.11.20210485.

Carbajo CN, da Veiga GL, Mota RT, Fonseca FLA, Lima VL. Implementation of risk stratification for the care of glaucoma patients during the resumption of in-person care during the COVID-19 pandemic – a preliminary study. Jhgd 2023;33:501–8. https://doi.org/10.36311/jhgd.v33.14480.

Abreu LC. Four Years into the Era of COVID-19: The Virus Persists and Global Vaccination Efforts Remain Quiescent”. Jhgd 2023;33(3):315-318. DOI: 10.36311/jhgd.v33.15423

José Natário A, Luciano da Veiga G, Loduca Lima V, Gascón T, Claudio dos Santos Pinheiro J, Regina Santos Raimundo J, et al. The influence of social isolation on the incidence of positivity in covid-19 tests in a metropolitan region of São Paulo, Brazil. Jhgd 2021;31:476–83. https://doi.org/10.36311/jhgd.v31.12656.

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Publicado

2024-04-11

Número

Vol. 34 Núm. 1 (2024)

Sección

ORIGINAL ARTICLES

Licencia

Derechos de autor 2024 Moreira NG, Bibo TA, Gaiatto ACM, Raimundo JRS, Encinas JFA, Alves BCA, Gascón TM, Fonseca FLA, Veiga G

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.